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You are here:Home » Antibodies » Abs to T Cells, Lymphocytes » Anti -T Cell Receptor Chain

Anti -T Cell Receptor Chain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
The T cell antigen receptor (TCR) is a multichain structure composed of an antigen binding heterodimer (Ti a/b) in an obligate, non-covalent association with the invariant CD3 g, d, e chains, and two CD3 z chains. The z chain exists in the TCR complex primarily as a disulfide-linked homodimer; however, in a minority of receptors, the z chain exists as a heterodimer with the homologous h chain. Expression of all seven chains (e exists in heterodimers with both g and d chains) is required for efficient cell surface expression of the TCR. It is the CD3 complex which is thought to couple receptor-ligand binding to downstream intracellular signaling events. In particular, the z chain and the CD3 e chain have been implicated in mediating TCR function. Analysis of the protein sequences present in the cytoplasmic domains of the z chain and the CD3 chains identified a common motif consisting of two YXXL-based sequences separated by six tyrosine activation motif. Three copies of to eight amino acids. This motif has been termed ITAM for intracellular tyrosine activation mofit. Three copies of the ITAM motif are present in each z chain, and single copies are present in each of the CD3-e,-g, and-d subunits. The ITAM sequences present in the z chain and the other CD3 subunits are required for interaction with downstream signaling molecules. Engagement of the TCR by antigen complexed to a MHC molecule activates a biochemical cascade which involves both protein tyrosine kinases (PTK) and tyrosine phosphatases, and which culminates in cytokine release, cellular differentiation, and cellular proliferation. The multichain TCR does not possess intrinsic kinase activity; however, at least three PTKs have been implicated in proximal TCR signaling events including: Fyn, Lck, and ZAP70. Fyn and Lck are members of the Srcfamily of protein tyrosine kinases while ZAP70 is a member of the Syk-family of protein tyrosine kinases. After engagement of the TCR, ZAP70 is phosphorylated on tyrosine residues and associates with the tyrosine phosphorylated TCR z and CD3 subunits. Tyrosine phosphorylation of TCR z and ZAP70, and their subsequent association is dependent upon the Lck and Fyn PTKs.
Catalog #T2018
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA(5): 0.11ug/ml
Immunoprecipitation/ IP-Kinase Assay(5): 5ug
Western Blot: 13ug/ml Note: To co-IP TCR z and ZAP70, prepare Jurkat cell lysates using 1% NP-40.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1.k
Clone No3G288
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide corresponding to the cytoplasmic domain of the human T Cell Receptor z (zeta) chain coupled to a protein carrier.
SpecificityDirected against human TCR z; however, it is also reactive with murine TCR z. Recognizes both the un-phosphorylated and phosphorylated forms of TCR z. Does not crossreact with the B Cell protein tyrosine kinase Syk. Reactivity has been confirmed by Western blot analysis of cell lysates from human Jurkat T cells. Additionally, this antibody is capable of co-precipitating the z chain and associated tyrosine phosphorylated protein ZAP-70. ZAP-70 is a protein tyrosine kinase which interacts with the TCR z chain via two SH2 domains located in the ZAP-70 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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