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You are here:Home » Antibodies » Antibodies-Transcription Factors » Anti -TCF-4 (T-cell Transcription Factor 4)

Anti -TCF-4 (T-cell Transcription Factor 4)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC
TCF-4, transcription factor 4, is a basic helix-turn-helix transcription factor. This protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG')-a motif first identified in immunoglobulin enhancers. The gene for TCF-4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described. TCF4 is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
Catalog #T2026
ApplicationsSuitable for use in Western Blot, ELISA, Supershift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5-2ug/ml. Detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4.
Supershift Assay: Reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA).
Immunoprecipitation: Reported to immunoprecipitate TCF-4 from SW620 whole cell lysates as a complex with b-Catenin.
Immunocytochemistry/Immunohistochemistry: Reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS.
Optimal dilutions to be determined by the researcher.
Positive ControlDLD1 (human colorectal adenocarcinoma), Cos-1 cells expressing transfected TCF-4 or HCT116 (human colorectal carcinoma) cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Clone TypeMonoclonal
Clone No0.T.149
FormSupplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman TCF-4 (aa31-331) (Accession# CAB97213.1)
SpecificityRecognizes human TCF-4 at ~66kD. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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