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You are here:Home » Cloning » Cloning-Vectors » TCF Reporter Plasmid (TOPflash, TK-luciferase reporter)

TCF Reporter Plasmid (TOPflash, TK-luciferase reporter)

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Specifications

Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash (F5950) containing mutated TCF binding sites is also available as a negative control.
Catalog #T2030
Size5.5kb
ApplicationsSuitable for use in TCF assays. Other applications not tested.
Recommeded DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PurityPurified by immunoaffinity chromatography.
FormSupplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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