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You are here:Home » Molecular Biology » MB-Telomeres » Telomerase, Human Control Peptide (EST2, hEST2, TCS1, Telomerase Catalytic Subunit, Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

Telomerase, Human Control Peptide (EST2, hEST2, TCS1, Telomerase Catalytic Subunit,
Telomerase-associated protein 2, Telomere Reverse Transcriptase, TERT, TP2, TRT)

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Specifications

Control Peptide for T2399-50 (antiserum) and T2399-45 (affinity purified)
Catalog #T2399-60
A 21aa Peptide sequence mapping in the middle of human Est2. Peptide has no significant sequence homology with the mouse Est2.
The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities. Most recently, introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling.
Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2 1122 aa; ~127kD). hEST2 was localized in the nucleus.
ApplicationsSuitable for use in ELISA for blocking experiments. Not suitable for Western Blot due to low MW: <3kD. Other applications not tested.
Recommended Dilution5-10ug/1ug of affinity pure antibody or 1ul antiserum
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
SourceHuman
PurityHighly purified
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide


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