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You are here:Home » Molecular Biology » Cloning-Enzymes » Terminal Deoxynucleotidyl Transferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT)

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Specifications

TdT is a template-independent DNA polymerase located in the cell nucleus which catalyses irreversible terminal addition, via polymerization, of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt’s lymphoma.
Catalog #T2750
Applications• Oligo and DNA 3’-terminal labeling
• Homopolymeric tailing of linear duplex DNA with any type of termini
Concentration
20-40u/ul
Storage Buffer
100mM potassium acetate, pH 6.8, 2mM 2-mercaptoethanol, 0.01% Triton X-100, 50% glycerol.
Reaction Buffer (5X)
1M potassium cacodylate, pH 7.2 (25°C), 5mM CoCl2, 0.5mM DTT, 0.05% Triton X-100
Unit Definition
1 unit incorporates 1 nanomoles of deoxythymidylate into a DE-81 absorbable form in 60 minutes at 37ºC.
Quality Control
Tested for absence of exo-, endodeoxyribonucleases and ribonucleases.
Alternate namesCalf thymus Supplied with Reaction Buffer


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