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You are here:Home » Antibodies » Antibodies-Proteins » Anti -Terminal Deoxynucleotidyl Transferase (TdT)

Anti -Terminal Deoxynucleotidyl Transferase (TdT)


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Clone Host Grade Applications
Polyclonal Rabbit Purified IH
TdT is a nuclear enzyme that catalyzes the random addition of deoxynucleotide triphosphate to the 3-OH groups of single-stranded DNA. A 58kD immunoreactive peptide has been detected in human and mouse lymphoblastoid cell lines. Also, a 58kD-60kD major peptide has been seen on Western Blots of mouse, rat, calf, feline and chicken thymus extracts. Elevated activity of TdT has been shown in neoplasms derived from immature lymphoid cells, lymphoblastic lymphomas (LL), acute lymphoblastic leukemias (ALL), lymphoid blast crisis of chronic myeloid leukemias (CML), and lymphomas. Rabbit anti-TdT may be used to distinguish LL from other lymphomas that do not express TdT.
Catalog #T2750-09
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Frozen and formalin-fixed paraffin-embedded human tissue sections, or cell smears. Epitope Retrieval Required, use Citrate buffer, pH 6.0.
Enzyme Digestion is not required.
Optimal dilutions to be determined by the researcher.
Positive ControlThymus
Expected Staining PatternNuclear
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
ImmunogenPurified TdT isolated from calf thymus.
SpecificityRecognizes Terminal Deoxynucleotidyl Transferase (TdT).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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