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You are here:Home » Antibodies » Abs to Homeobox (HOX) » Anti -TGF beta-induced Factor, CT (Homeobox, TGIF, TG-interacting Factor, 5'-TG-3' Interacting Factor)

Anti -TGF beta-induced Factor, CT (Homeobox, TGIF, TG-interacting Factor, 5'-TG-3'
Interacting Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP
Novel homeobox gene, denoted TGIF (5"TG3' interacting factor), belongs to an expanding TALE (three amino acid loop extension) superclass of atypical homeodomains. The TGIF homeodomain binds to a previously characterized retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE), which contains an unusual DNA target for a homeobox (1). In vitro studies have implicated TGIF as a transcriptional repressor and corepressor in retinoid and TGF-beta signaling pathways that regulate several important biological processes. Heterozygous nonsense and missense mutations of the human TGIF gene have been associated with holoprosencephaly, the most common congenital malformation of the forebrain (2). It has been reported that TGIF plays an unexpected role in the inhibition of Smad2 phosphorylation, which occurs by a mechanism independent of its association with Smad2. This inhibitory function of TGIF is executed in concert with c-Jun, which facilitates the interaction of TGIF with cPML, resulting in the nuclear sequestration of cPML and the disruption of the cPML-SARA complex (3).
Catalog #T3630-01C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E586
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human TGIF.
SpecificityRecognizes human TGIF, CT. Detects both phosphorylated and unphosphorylated TGIF.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names5-TG-3-interacting factor 1


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