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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IC FC

The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack.The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Brefeldin A causes the contents of the golgi stack to be redistributed into the endoplasmatic reticulum while the TGN collapse upon the microtubule organizing center. This makes immunofluorescence detection of TGN38, following Brefeldin A treatment, a useful tool for the discrimination of the TGN from the Golgi stack.
Catalog #	T3660-05A
Applications	Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
	Immunocytochemistry: 1:1:000
	Western Blot: 1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability
	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D794
Host	Mouse
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Ascites
Immunogen	Synthetic peptide corresponding to aa 1-17 (L1PSASKPNNTS
SENNPP17) of N-terminal rat TGN38 (trans-Golgi network 38).
Specificity	Recognizes the luminal domain of rat TGN38. Detects a band of ~38kD by Western blot, representing deglycosylated TGN38. Under normal Western blot conditions detects a band of ~85-95kD depending on cell type. Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.