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Specifications

Clone Host Grade Applications Polyclonal Sheep Serum E B IH IF

T3660-06 recognizes a glycoprotein designated TGN38 that is found primarily in the Trans-golgi network, and has been found to be an excellent marker for this cellular organelle. TGN38 is likely to have a role in intracellular transport. It is the homologue of human TGN46.
Catalog #	T3660-06
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence (IC). Other applications not tested.
Recommended Dilutions	Immunofluorescence (IC): 1:100. Fixation with 3% paraformaldehyde or methanol is recommended
	and incubation for 60 minutes at RT in blocking buffer (0.1% gelatin in TBS/triton)
	Western Blot: 1:500-1:1000. 5% milk/TBS (+ 0.1% Tween-20) and incubation for 60 minutes at RT
	Immunohistochemistry (Paraffin):1:100-1:200. Fixation with methanol or methanol/acetone recommended
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Rat
Concentration	Not Determined
Form	Supplied as a liquid, pH 7.2, 1% BSA, 0.09% sodium azide.
Purity	Serum
Immunogen	Recombinant fusion protein corresponding to extracellular domain of TGN38.
Specificity	Recognizes rat TGN38. TGN38 is a heavily glycosylated protein of around 80-90kD. Species Crossreactivity: mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.