| The lysine analog, thialysine, has been implicated in the mechanism for the inhibitory effect of the on human acute leukemia Jurkat T cells. When Jurkat T cells were treated with thialysine (0.32-2.5mM), apoptotic cell death along with several biochemical events such as mitochondrial cytochrome c release, caspase-9 activation, caspase-3 activation, degradation of poly (ADP-ribose) polymerase, and DNA fragmentation was induced in a dose- and time-dependent manner. However, these thialysine-induced apoptotic events were significantly abrogated by an ectopic expression of Bcl-xL, which is known to block mitochondrial cytochrome c release. Decylubiquinone, a mitochondrial permeability transition pore inhibitor, also suppressed thialysine-induced apoptotic events. Comparison of the thialysine-induced alterations in the cell cycle distribution between Jurkat T cells transfected with Bcl-xL gene (J/Bcl-xL) and Jurkat T cells transfected with vector (J/Neo) revealed that the apoptotic cells were mainly derived from the cells accumulated in S and G2/M phases following thialysine treatment. The interruption of cell cycle progression in the presence of thialysine was accompanied by a significant decline in the protein level of cdk4, cdk6, cdc2, cyclin A, cyclin B1, and cyclin E. These results demonstrate that the cytotoxic activity of thialysine toward Jurkat T cells is attributable to not only apoptotic cell death mediated by a mitochondria-dependent death signaling pathway, but also interruption of cell cycle progression by a massive down-regulation in the level of cdks and cyclins. |
|
| Catalog # | T5000 |
| CAS Number | 4099-35-8 |
| Molecular Formula | C5H12N2O2S·HCl |
| Molecular Weight | 200.70 |
| Assay by TLC | |
| >99% |
| Appearance | |
| Off-white powder |
| Solubility (H2O, 50mg/ml) | |
| Light tan, clear, complete |
| Assay (Elemental Analysis) | |
| ~81% based on Nitrogen content |
| Assay (Elemental Analysis) | |
| N ~13% |
| Methanol/Chloroform/Ammonia | |
| UV, Chlorine-Tolidine (2/2/1) |
| ~17% (2/2/1) |
| Isopropanol/Ammonia Conc. | |
| 1/1 |
| n-Butanol/Acetic Acid/H2O | |
| 4/2/2 |
| Plate | |
| Silicagel 60 F254 |
| Chloride Content | (Titration) |
| ~17% (AgNO3) |
| Melting Point | |
| ~201°C |
| Specific Rotation (a,24 D) | |
| As reported (1% in water) |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | H-Cys(aminoethyl)-OH?HCl; L-4-Thialysine hydrochloride |
