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You are here:Home » Antibodies » Abs to Vitamins » Anti -Thiamine, Conjugated

Anti -Thiamine, Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rat Purified E IH
Thiamin is one of the B vitamins, a group of water-soluble vitamins that are part of many of the chemical reactions in the body. Thiamin (vitamin B1) helps the body's cells convert carbohydrates into energy. It is also essential for the functioning of the heart, muscles, and nervous system.
Catalog #T5004
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 1:500-1:1000. Immunoreactive fibers located in the pulvinar nucleus of monkey brain. Also, immunoreactive fibers located between the pulvinar and caudate nucleus of monkey brain.
Optimal dilutions to be determined by the researcher.
SpecificityUsing a conjugate Thiamine-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
Compound Cross-reactivity ratio (a) Thiamine-BSA 1 Folic acid-BSA 1/>80 Thioctic acid-BSA 1/>50,000
(a) Thiamine-BSA concentration/unconjugated or conjugated compound concentration at half displacement.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRat
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O.
PurityPurified
ImmunogenSynthetic Thiamine, conjugated to BSA.
SpecificityRecognizes protein-linked Thiamine. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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