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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and they are ubiquitous in essentially all living cells. Thioredoxin is a 12kD redox protein that has powerful protein disulfide oxidoreductase activity. It has been shown to be involved in various cellular processes, including activation of the transcription factor NR-KB.
Catalog #	T5010-02
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 0.01-0.1ug/ml
	Western Blot: 1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Hybridoma	Sp2/0 /AG14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8A258
Host	Mouse
Source	Human
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant human Thioredoxin produced in E. coli
Specificity	Specific for the thioredoxin fusion protein from human. Does not crossreact with thioredoxin from E. coli.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.