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You are here:Home » Antibodies » Abs to Thioredoxin » Anti -Thioredoxin

Anti -Thioredoxin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the N- or C-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enable a selective identification and purification of the protein of interest. Eukaryotic genes are often cloned into E. coli thioredoxin gene, resulting in the expression of a desired protein as a fusion hybrid with thioredoxin. Thioredoxin is a small electron transport protein that serves as hydrogen door in the enzymatic reduction of ribonucleotides to deoxyribonucleotides. Thioredoxin from E. coli consists of a single polypeptide of 108aa (~11.7kD). The gene encoding Thioredoxin has been clones into several plasmids and serves as a fusion partner for the expression of other clones genes. The system often produces high expression of soluble fusion proteins in the E. coli cytoplasm. The fusion proteins may also fold correctly with biological activity. Anti-Thioredoxin antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysate, using immunoaffinity chromatography or used in immunoprecipitation. Anti- thioredoxin can also be used for the immunocytochemical detection of thioredoxin in cells and tissues that express transfected bacterial thioredoxin gene. Ant-thioredoxin may be used in various immunoassays to identify the expression of thioredoxin fusion protein.
Catalog #T5010-02B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaSP2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8A263
HostMouse
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 100ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified recombinant corresponding to the Trx-tag fusion protein
SpecificityRecognizes the Thioredoxin fusion protein from E. coli and human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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