Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant E B IH

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase. Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction. A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation. Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1. Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury.
Catalog #	T5010-10H
Applications	Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
	Recommended Dilutions:
	Western Blot: 1:1000
	Immunohistochemistry (Paraffin): 1:400. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Molecular Weight	12kD
Clone Type	Monoclonal
Isotype	IgG
Clone No	7H76
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to residues surrounding Ala9 of human Thioredoxin 1.
Specificity	Recognizes endogenous levels of total human Thioredoxin 1 at ~12kD. Species Crossreactivity: mouse and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.