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You are here:Home » Antibodies » Antibodies-Thioredoxin » Anti -Thioredoxin Reductase 1 (TrxR1)

Anti -Thioredoxin Reductase 1 (TrxR1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
Catalog #T5010-14A
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1-2ug/ml of this lot detected thioredoxin reductase 1 in RIPA lysates from rat brain microsomal preparation and HeLa cells.
Immunocytochemistry: Has been reported by an independent laboratory to detect thioredoxin reductase 1 in U937 cells stained with DAPI and CMXRos.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponding to aa137-150 (C-PHRIKATNNKGKEK) of human thioredoxin reductase 1
SpecificityRecognizes thioredoxin reductase 1, Mr 58kD. An additional protein was also detected, Mr ~70kD, that was inhibited by the immunizing peptide. Species Crossreactivity: Rat and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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