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You are here:Home » Molecular Biology » Cells-Extracts, Lysates » THP-1 Nuclear Extract, PMA stimulated (Human Monocyte)

THP-1 Nuclear Extract, PMA stimulated (Human Monocyte)

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Specifications

Permanent murine cell lines of fast and slow skeletal myocyte derivation.
Catalog #T5019-01A
Nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with PMA (10ng/ml).
Storage and Stability For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
PurityExtracts have been quality control tested by Western blot.
Concentration~2.5mg/ml
FormSupplied as a liquid in 20mM Hepes pH 7.5, 400mM NaCl, 20% glycerol, 0.1 mM EDTA, 10mM NaF, 10uM Na2MoO4, 1mM NaVO3, 10mM PNPP, 10mM beta-glycerophosphate, 1mM DTT and protease inhibitors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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