Suitable for use in Flow Cytometry, Direct ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Flow Cytometry: 2.5ug/ml; 10ul labels 1x10e6 bEnd.3 cells Western Blot: 1ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Thrombomodulin is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Immunohistochemistry: 8-25ug/ml detects Thrombomodulin in frozen sections of normal mouse kidney and heart. Immunocytochemistry: 8-25ug/ml. Used with bEnd.3 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Between each step, cells were washed with PBS, BSA. Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Leu17-Ser517 from mouse Thrombomodulin expressed in NSO cells (P15306).

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Recognizes recombinant and native mouse Thrombomodulin. Does not crossreact with recombinant human Thrombomodulin.