| Thrombomodulin is a 110-120kD, endothelial cell-specific type I transmembrane glycoprotein. It binds thrombin, rendering it unavailable for clotting, and activates protein C, which also serves as an anticoagulant. Mouse Thrombomodulin is 577aa in length. It contains a 510aa extracellular domain (ECD) (aa17-517) that shows one C-type lectin domain (aa31-167) and six EGF-like regions (aa240-480). Thrombin binds to aa403-480. One isoform may exist that shows a deletion of the C-terminal 86 amino acids (aa492-577). Over aa17-517, mouse Thrombomodulin is 66%, 85% and 67% aa identical to human, rat and canine TM, respectively. |
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| Catalog # | T5048-04 |
| Applications | Suitable for use in Flow Cytometry, Direct ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 bEnd.3 cells in a total reaction volume <200ul. |
| Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse Thrombomodulin is ~5ng/well. |
| Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Thrombomodulin is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold. |
| Immunohistochemistry: 25ug/ml detects Thrombomodulin in frozen sections of normal mouse kidney and heart. |
| Immunocytochemistry: 10ug/ml. Used with bEnd.3 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Between each step, cells were washed with PBS, BSA. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 8H203 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant protein corresponding to aa17-517 from mouse Thrombomodulin expressed in NSO cells (P15306). |
| Specificity | Recognizes recombinant mouse Thrombomodulin. Does not crossreact with recombinant human Thrombomodulin. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | TM, Fetomodulin, CD141 |
