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You are here:Home » Antibodies » Abs to Thrombopoietin » Anti -Thrombopoietin (TPO, Mpl-ligand, MGDF)

Anti -Thrombopoietin (TPO, Mpl-ligand, MGDF)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
TPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. Human TPO is a soluble 18.5kD protein containing 174 amino acid residues which comprises the receptor binding domain of the Mpl-ligand protein.
Catalog #T5050-06E
ApplicationsSuitable for use in Indirect ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.2ug/ml detects a single band at ~46kD.
Indirect ELISA: 50ng will yeild an OD of >2.0 under standard assay conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No7C200
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 10mg/ml BSA.
PurityPurified
ImmunogenRecombinant human TPO
SpecificityRecognizes recombinant human TPO and TPO (244-270). Does not react with the following human proteins: recombinant EPO, ACTH (1-39), Calcitonin, Insulin, Insulin-like Growth Factor 1, Insulin-like Growth Factor 2 and Parathyroid Hormone.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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