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You are here:Home » Antibodies » Abs to Thrombopoietin » Anti -Thrombopoietin (TPO, Mpl-ligand, MGDF)

Anti -Thrombopoietin (TPO, Mpl-ligand, MGDF)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B N
TPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. Human TPO is a soluble 18.5kD protein containing 174 amino acid residues which comprises the receptor binding domain of the Mpl-ligand protein.
Catalog #T5050-07
Applications  
Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended DilutionNeutralilzation: 0.05-0.08ug/ml is required to yield one half maximal inhibition (ND50) of the biological activity of hTPO, 5ng/ml.
ELISA (Indirect and Sandwich): 0.5-2ug/ml (100ul/well) allows the detection of at least 0.2-0.4ng/well of recombinant hTPO used in conjunction with compatible secondary reagents.
ELISA (Sandwich): Detection antibody: T5050-08
Western Blot: 0.1-0.2ug/ml detects 1.5-3ng/lane hTPO, when used with compatible secondary reagents, under reducing and non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from a sterile- ltered PBS, pH 7.2. Reconstitute with sterile 40-50?glycerol, dH2O to 0.1-1mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant hTPO (human TPO)
SpecificityRecognizes human thrombopoietin (TPO, Mpl-ligand, MGDF).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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