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You are here:Home » Molecular Biology » Growth Factors-Thrombopoietin » Thrombopoietin, Recombinant, Mouse (TPO, Mpl-ligand, MGDF)

Thrombopoietin, Recombinant, Mouse (TPO, Mpl-ligand, MGDF)

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Specifications

Recombinant Murine TPO is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18.7kD which comprises the receptor binding domain of the Mpl-ligand protein.
Catalog #T5050-12
Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Val-Ala-Pro.
Biological ActivityRecombinant TPO is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of MO7e cells is less then 1 ng/ml, corresponding to a Specific Activity of 1x10e6 IU/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight18.7kD
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. No additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of 100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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