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You are here:Home » Antibodies » Abs to Thrombopoietin » Anti -Thrombopoietin Receptor (c-Mpl) (TPO)

Anti -Thrombopoietin Receptor (c-Mpl) (TPO)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95kD type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily.
Catalog #T5050-50
ApplicationsSuitable for use in Western Blot, Immnohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.25-1ug/ml detects TPO receptor in RIPA lysates from DAMI cells. DAMI cell lysate was resolved by
electrophoresis, transferred to PVDF and
probed with T5050-50. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: Stains megakaryocytes in bone marrow biopsy sections.
Immunoprecipitation: 5ug immunoprecipitates TPO receptor from 500ug of DAMI RIPA lysate.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 615-628, C-ANHSYLPLSYWQQP, of human Thrombopoietin Receptor, conjugated to KLH. Species Sequence Homology: mouse, 100%
SpecificityRecognizes glycosylated and unglycosylated human TPO receptor/c-Mpl, Mr 70kD. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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