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You are here:Home » Antibodies » Abs to Thrombopoietin » Anti -Thrombopoietin Receptor (c-Mpl) (TPO)

Anti -Thrombopoietin Receptor (c-Mpl) (TPO)

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Specifications

Clone Host Grade Applications
Rabbit Affinity Purified B IP IH
Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95kD type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily.
Catalog #T5050-50
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.25-1ug/ml detects TPO receptor in RIPA lysates from DAMI cells. DAMI cell lysate was resolved by
electrophoresis, transferred to PVDF and
probed with T5050-50. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: Stains megakaryocytes in bone marrow biopsy sections.
Immunoprecipitation: 5ug immunoprecipitates TPO receptor from 500ug of DAMI RIPA lysate.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type


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