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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Catalog #	T5100-06A
Applications	Suitable for use in Western Blot. Not suitable for Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot (reduced and non-reduced): 0.5-1ug/ml for 2hrs at RT.
	Optimal dilution to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3F350
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5100-06AX
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified human TSP. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix.
Specificity	Recognizes human Thrombospondin (TSP) at ~450kD (non-reduced) 170 -180kD (reduced). It shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.