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You are here:Home » Antibodies » Abs to Thrombospondin » Anti -Thrombospondin (TSP)

Anti -Thrombospondin (TSP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Catalog #T5100-06D
ApplicationsSuitable for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native verified) (Use Protein G): 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:25 to 1:50 for 60 min at RT
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.
MW of Antigen ~450kD (non-reduced); 170 to 180kD (reduced)
EpitopeCollagen Type V-binding domain of TSP
Positive ControlPlatelets in placenta, megakaryocytes in bone marrow.
Cellular LocalizationSecretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F353
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5100-06DX
PurityPurified by Protein G affinity chromatography.
ImmunogenReduced and alkylated puri ed human TSP (fully denatured) from the supernatant of thrombin-activated platelets
SpecificityRecognizes human TSP. Inhibits TSP-collagen interaction. Its binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i.e. at low conc. of Ca2+. It shows no crossreactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild crossreaction with TSP 2. Species Crossreactivity: Bovine, porcine, equine, canine, sheep, mouse, and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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