Anti -Thrombospondin (THBS, TSP, Thrombospondin 1, THBS1, THBS-1, Tsp1, Tsp-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IP IH IF |
|
| Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. | | | Catalog # | T5100-06H | | Applications | Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:25-1:50. Using an ABC Method. Incubation time is 30 minutes at RT. | | Immunohistochemistry (Paraffin): 1:25-1:50. Requires high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to staining. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Tonsil or placenta | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9F208 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid, sodium azide. | | Purity | Purified | | Immunogen | BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum | | Specificity | Recognizes the non-reduced form of human Thrombospondin at~450kD and the reduced form at ~170-180kD. | | Species Crossreactivity | Bovine, porcine, equine, canine, sheep, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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