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You are here:Home » Antibodies » Abs to Thrombospondin » Anti -Thrombospondin (THBS, TSP, Thrombospondin 1, THBS1, THBS-1, Tsp1, Tsp-1)

Anti -Thrombospondin (THBS, TSP, Thrombospondin 1, THBS1, THBS-1, Tsp1, Tsp-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH IF
Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Catalog #T5100-06H
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:25-1:50. Using an ABC Method. Incubation time is 30 minutes at RT.
Immunohistochemistry (Paraffin): 1:25-1:50. Requires high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to staining.
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil or placenta
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9F208
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid, sodium azide.
PurityPurified
ImmunogenBALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum
SpecificityRecognizes the non-reduced form of human Thrombospondin at~450kD and the reduced form at ~170-180kD.
Species CrossreactivityBovine, porcine, equine, canine, sheep, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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