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You are here:Home » Antibodies » Abs to Thrombospondin » Anti -THBS1 (Thrombospondin-1, TSP1, TSP) (Biotin)

Anti -THBS1 (Thrombospondin-1, TSP1, TSP) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Catalog #T5100-06P
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.1-0.4ug/ml.
Western Blot: 0.1-0.2ug/ml. The detection limit for rhThrombospondin-1 is approximately 50 ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, NS0-derived, recombinant human Thrombospondin-1.
SpecificityRecognizes human Thrombospondin-1. Crossreactivity: Thrombospondin 2 and Thrombospondin 4 (less than 5%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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