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You are here:Home » Antibodies » Abs to Thrombospondin » Anti -Thrombospondin (TSP)

Anti -Thrombospondin (TSP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Thrombospondin is a protein from platelet agranules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. This antibody inhibits TSP collagen interaction. Its binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate, and heparin). It shows no cross reaction with fibronectin, fibrinogen, and von Willebrand factor. It shows a mild cross reactivity towards TSP 2.
Catalog #T5100-06T
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hrs
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil, placenta
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2D7
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenReduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
SpecificityRecognizes human thrombospondin. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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