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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH FC IF

Thrombospondin is a protein from platelet agranules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. This antibody inhibits TSP collagen interaction. Its binding to TSP is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate, and heparin). It shows no cross reaction with fibronectin, fibrinogen, and von Willebrand factor. It shows a mild cross reactivity towards TSP 2.
Catalog #	T5100-06T
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunoprecipitation: 2ug/mg protein lysate
	Western Blot: 1-2ug/ml for 2hrs
	Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
	Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
	Optimal dilutions to be determined by the researcher.
Positive Control	Tonsil, placenta
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	2D7
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Specificity	Recognizes human thrombospondin. Species Crossreactivity: bovine, porcine, equine, canine, sheep, mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.