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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IC FC

Catalog #	T5145-03
Applications	Suitable for use in Flow Cytometry and Complement Cytotoxicity. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: 1:5
	Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents	Rat anti mouse IgM heavy chain: FITC
Hybridoma	Spleen cells from immunized CBA mice were fused with cells of the mouse NS1 myeloma cell line.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	3H2731
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS with 0.09% sodium azide.
Purity	Purified by ammonium sulfate precipitation.
Immunogen	Xenogeneic rat thymocytes.
Specificity	This antibody has been used to selectively remove cytotoxic T cells or their precursors, helper T cells and alloantigen activated proliferating T cells. Thy 1.1 is not present on T cells of most inbred mouse strains. In rats Thy1.1 is expressed on fibroblasts and bone marrow cells, but not on peripheral blood lymphocytes. This clone is cytotoxic with rabbit guinea pig complement.
Species Crossreactivity	Recognizes both mouse and rat Thy1.1
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.