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You are here:Home » Antibodies » Antibodies-T Cells, Lymphocytes » Anti -Thy 1.1

Anti -Thy 1.1


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Clone Host Grade Applications
Monoclonal Mouse Purified IC FC
Catalog #T5145-03
ApplicationsSuitable for use in Flow Cytometry and Complement Cytotoxicity. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1:5
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsRat anti mouse IgM heavy chain: FITC
HybridomaSpleen cells from immunized CBA mice were fused with cells of the mouse NS1 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3H2731
FormSupplied as a liquid in PBS with 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenXenogeneic rat thymocytes.
SpecificityThis antibody has been used to selectively remove cytotoxic T cells or their precursors, helper T cells and alloantigen activated proliferating T cells. Thy 1.1 is not present on T cells of most inbred mouse strains. In rats Thy1.1 is expressed on fibroblasts and bone marrow cells, but not on peripheral blood lymphocytes. This clone is cytotoxic with rabbit guinea pig complement.
Species CrossreactivityRecognizes both mouse and rat Thy1.1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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