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You are here:Home » Antibodies » Abs to Oxidative Products and Metabolites » Anti -Thymine Dimer (Cyclobutane Pyrimidine Dimer, CPD)

Anti -Thymine Dimer (Cyclobutane Pyrimidine Dimer, CPD)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC Gel
This antioody is intended for the detection of DNA damage as a result of UV irradiation.
Catalog #T5151-09
HybridomaMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) spleen cells
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, Southern Blot, Northern Blot, and Dot Blot. Other applications have not been tested.
Recommended DilutionIn situ Hybridization: 1:40-1:80. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended.
Southern, Northern and Dot Blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes used for in situ. (reported to work, but no data is available)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoKTM53
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS containing 0.1% sodium azide and protein stabilizer
PurityPurified by Protein A affinity chromatography
ImmunogenUV-irradiated salmon sperm DNA
SpecificityRecognizes thymine dimers produced by UV irradiation in double- or single- stranded DNA. Does not react with (6-4) photo products.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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