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You are here:Home » Antibodies » Abs to T Cells, Lymphocytes » Anti -Tim3 (T Cell Immunoglobulin and Mucin Domain Containing Protein 3)

Anti -Tim3 (T Cell Immunoglobulin and Mucin Domain Containing Protein 3)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
Tim-3 is expressed on differentiated Th1 cells, but not on Th2 cells. It enhances macrophage activation, inhibits Th1-mediated auto- and alloimmune responses and promotes immunological tolerance. Galectin-9 is a ligand for Tim-3 and induces Th1 cell death. Signalling through the Tim-3 pathway may therefore negatively regulate Th1 responses.
Catalog #T5469-92D
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:20 10ul labels 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies Rabbit Anti Rat IgG HRP Rabbit Anti Rat IgG FITC Rabbit Anti Rat IgG RPE Rabbit Anti Rat IgG HRP Goat Anti Rat IgG FITC Goat Anti Rat IgG (MOUSE ADSORBED) DyLight™488, DyLight™549, DyLight™649 Goat Anti Rat IgG HRP Goat Anti Rat IgG RPE Goat Anti Rat IgG Biotin, FITC Goat Anti Rat IgG Alk. Phos.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9F94
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
SpecificityRecognizes mouse T-cell immunoglobulin and mucin domain-containing protein 3 (Tim-3), a member of the Tim family type I membrane proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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