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You are here:Home » Antibodies » Abs to T Cells, Lymphocytes » Anti -Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)

Anti -Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin
Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2,
HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
T cell immunoglobulin mucin-3 (Tim3) is a member of the T cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. Tim3 is selectively expressed on Th1 cells and interacts with galectin-9. It negatively regulates Th1 responses and affects macrophage activation. The 280aa mature human Tim3 contains a V-type Ig-like domain that shows multiple polymorphisms, followed by a mucin-like domain in the 171aa extracellular region, which shares 60% aa identity with the mouse Tim3 ECD. One splice variant is truncated within the mucin domain and presumably is secreted.
Catalog #T5469-92F
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 10ul labels up to 1x10e6 cells. Validated using whole blood monocytes.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of T5469-92F to up to 1x10e6 cells and incubate for 30 minutes at RT
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog #F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2A antibody.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for at 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2A
Clone No9L856
HostRat
SourceHuman
Concentration~0.01mg/ml
FormSupplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa22-200 from the extracellular domain of human Tim3 expressed in NSO cells (NP_116171).
SpecificityRecognizes human Tim3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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