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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -TINAG (Tubulointerstitial Nephritis Antigen, TIN-Ag)

Anti -TINAG (Tubulointerstitial Nephritis Antigen, TIN-Ag)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
TIN-Ag (Tubulointerstitial nephritis antigen) is a 48-58kD glycoprotein member of the peptidase C1 family of molecules. It is secreted by renal tubule epithelium and (presumably) small intestine columnar epithelium. TIN-Ag is an integral component of the renal tubule basement membrane (BM), and appears to promote proper BM matrix organization, block laminin polymerization, and serve as a receptor for the epithelial integrins a3b1 and avb3. Epithelial dissociation from the BM is associated with an epithelial-to-mesenchymal transition. Human TIN-Ag is synthesized as a 476aa preproprecursor. It contains a 19aa signal sequence, a 30aa furin-cleavable prosegment (aa20-49), and a 427aa mature region (aa50-476). Within the mature region is an SMB domain (aa61-106), one vWFC domain (aa119-154), and a nonenzymatic peptidase C1A region (aa218-466). Multiple splice variants are possible, and potential isoforms may show an alternative start site at aa322, or a combined deletion of aa119-169 plus 209-300, or a combination of an alternative start site at aa19 coupled to a 13aa substitution for aa209-476. Over aa20-476, human proTIN-Ag shares 86aa identity with mouse proTIN-Ag; over the mature region (aa50-476), human TIN-Ag shares 88aa identity with mouse TIN-Ag.
Catalog #T5475-50
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa20-476 from human TIN-Ag expressed in CHO cells (Q6NSC1).
SpecificityRecognizes human TIN-Ag. Species sequence homology: human TINAGL1 (1%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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