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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -TICAM1 (TIR Domain-containing Adapter Molecule 1, TICAM-1, Proline-rich, Vinculin and TIR Domain-containing Protein B, Putative NF-kappa-B-activating Protein 502H, Toll-interleukin-1 Receptor Domain-containing Adapter Protein Inducing Interferon beta, TIR

Anti -TICAM1 (TIR Domain-containing Adapter Molecule 1, TICAM-1, Proline-rich, Vinculin
and TIR Domain-containing Protein B, Putative NF-kappa-B-activating Protein 502H,
Toll-interleukin-1 Receptor Domain-containing Adapter Protein Inducing Interferon beta,
TIR

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
TRIF (TIR domain-containing adaptor inducing IFN-b; also TICAM1) is a 105-110kD cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF-3, -4, and -7, as well as NFkB and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712aa in length and contains three TRAF6 bonding motifs (aa84-91, 248-255 and 299-309), one TIR domain (aa390-460), a Pro-rich region (aa614-678), and an overlapping RHIM domain (aa661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23aa substitution for aa31-162 accompanied by a Pro substitution for aa633-660, a second shows a 44aa substitution for aa352-712, and a third shows a 38aa substitution for aa271-712. Over aa29-204, human TRIF shares 62% aa identity with mouse TRIF.
Catalog #T5498-51E
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:0.25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um-filtered solution in PBS, 5% trehalose. Reconstitute with 100ul PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived, recombinant human TIR domain-containing adaptor inducing IFN-b (aa29-204; Accession #Q8IUC6).
SpecificityRecognizes human TRIF. Species sequence homology: mouse (62%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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