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Abs to Receptors, Tyrosine Kinase
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Anti -TIE1 (TIE, Tyrosine-protein Kinase Receptor, Tunica Internal Endothelial Cell Kinase)
Anti -TIE1 (TIE, Tyrosine-protein Kinase Receptor, Tunica Internal Endothelial Cell Kinase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Tie-1 (human 1138aa; mouse 1134aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains. | | | Catalog # | T5498-65 | | Applications | Suitable for use in Western Blot and ELISA. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-10ug/ml using ECL. NIH/3T3 cells can be used as positive control. The predicted size of Tie-1 is ~135-140kD. | | ELISA:1:10,000- 1:100,000 using 50-100ng of control peptide per well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | 1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, and 0.05% sodium azide. | | Purity | Purified by affinity chromatography. | | Immunogen | 20 amino acid peptide sequence within the cytoplasmic, C-terminus of human Tie-1. | | Specificity | Recognizes human Tie-1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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