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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -TIE1 (TIE, Tyrosine-protein Kinase Receptor, Tunica Internal Endothelial Cell Kinase)

Anti -TIE1 (TIE, Tyrosine-protein Kinase Receptor, Tunica Internal Endothelial Cell
Kinase)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Tie-1 (human 1138aa; mouse 1134aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.
Catalog #T5498-65
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-10ug/ml using ECL. NIH/3T3 cells can be used as positive control. The predicted size of Tie-1 is ~135-140kD.
ELISA:1:10,000- 1:100,000 using 50-100ng of control peptide per well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, and 0.05% sodium azide.
PurityPurified by affinity chromatography.
Immunogen20 amino acid peptide sequence within the cytoplasmic, C-terminus of human Tie-1.
SpecificityRecognizes human Tie-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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