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You are here:Home » Antibodies » Antibodies-Receptors, Tyrosine Kinase » Anti -TIE1 (TIE-1, Tyrosine Protein Kinase Receptor, Tunica Internal Endothelial Cell Kinase)

Anti -TIE1 (TIE-1, Tyrosine Protein Kinase Receptor, Tunica Internal Endothelial Cell


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Mice lacking TIE 1 or TIE 2 are lethal. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis. TIEs appear to be acting downstream of the VEGFRs. Tie-1 (human 1138 AA; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.
Catalog #T5498-65F
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:100,000; using 50-100ng of control peptide/well. T5498-65E.
Western Blot: 1-10ug/ml using ECL technique. T5498-65D or NIH/3T3 cells can be used as positive control. Tie-1 ~135-140kD. Recombinant Tie-1 fusion protein migrate as 130-140kD due to glycosylation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
ImmunogenRecombinant human TIE1 protein (22-760aa, EC domain) was expressed as His-tagged Fc protein Chimera (> 90%)
SpecificityRecognizes human TIE1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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