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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Tie2, phosphorylated (Tyr1102) (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Anti -Tie2, phosphorylated (Tyr1102) (Tunica Internal Endothelial Cell Kinase,
Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Tie2 (also known as TEK) is an angiogenic receptor tyrosine kinase required for the later stage of blood vessel maturation. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation of Tie2 at Y1102 results in the activation of PI 3-kinase.
Catalog #T5498-70D
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested
Recommended DilutionsWestern Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml detected Tie2 in immersion fixed paraffin embedded sections of mouse embryonic brain and human placenta.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic Phosphopeptide corresponding to human Tie-2 Y1102 site.
SpecificityRecognizes human Tie-2 when phosphorylated at Y1102.
Species CrossreactivityMouse when phosphorylated at Y1100.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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