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You are here:Home » Antibodies » Abs to Angiopoietin » Anti -Tie2 (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Anti -Tie2 (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek,
Tyrosine Protein Kinase Receptor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Mice lacking TIE 1 or TIE 2 are lethal. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis. TIEs appear to be acting downstream of the VEGFRs. Tie-2 (human 1124 AA; mouse 1122 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells and their progenitors, angioblasts. It is also found in placenta and lung, with lower levels in umbilical vein endothelial cells, brain and kidney. Tie-2 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2-Ig-like C2-type, and 3-EGF-like domains.
Catalog #T5498-71J
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1:100,000
Western Blot: 1-10ug/ml (ECL)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% BSA
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide corresponding to 18aa, near the N-terminal, extracellular of human Tie2, conjugated to KLH. Species Sequence Homology: mouse and rat; 100%, bovine; 94%.
SpecificityRecognizes human Tie2. No significant sequence homology is seem with Tie1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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