| TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. |
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| Catalog # | T5498-72 |
| Applications | Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Immunoprecipitation: Reported to immunoprecipitate Tie2/TEK. |
| Western Blot: 0.2-1ug/ml. HUVEC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T5498-72. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. |
| Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections. Not suitable for use in paraf n- embedded sections. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | |
| HUVEC cell lysate |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 4i64 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Fusion protein corresponding to aa1-745 of human Tie2/TEK with a C-terminal His6-tag expressed in infected SF-9 cells (NM_000459). |
| Specificity | Recognizes human Tie2/TEK, Mr 145kD. Species Crossreactivity: mouse, rat and porcine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
