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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Tie2 Receptor (Tunica Internal Endothelial Cell Kinase Receptor, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Anti -Tie2 Receptor (Tunica Internal Endothelial Cell Kinase Receptor, Angiopoietin1
Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP FC
Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells and critical for vasculogenesis during development and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis. The ligand for Tie has yet to be identified. A family of ligands known as the angiopoietins have recently been shown to bind Tie2. Interestingly, these ligands appear to have opposing actions as Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2, whereas Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation. The phosphorylated Tie2 associates with different downstream signaling components. Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase and p56Dok-2 can interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains.
Catalog #T5498-80
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 5ug/ml, detects 20ng recombinant Tie-2. Recognizes a band at ~140kD
Flow Cytometry: 10ug/ml
Immunoprecipitation: 10ug/ml of lysate
Optimal dilutions to be determined by the researcher.
Positive ControlPlacenta and lung tissue
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2Q228
FormSupplied as a liquid in PBS, pH 7.4, 0.05% Bronidox as preservative, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenThe extracellular domain of mouse Tie2 receptor.
SpecificityRecognizes mouse Tie2 Receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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