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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)

Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP
TFPI-2 is a secreted protein that inhibits several serine proteases.
Catalog #T5576-21
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml with appropriate secondary reagents detects mouse TFPI-2. The detection limit for recombinant mouse TFPI-2 is ~5ng/well.
Western Blot: 1-2ug/ml with the appropriate secondary reagents detects mouse TFPI-2. Using a colorimetric detection system, the detection limit for recombinant mouse TFPI-2 is ~10ng/lane under non-reducing conditions. Reducing conditions are not recommended for use.
Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant mouse TFPI-2 from conditioned cell culture medium.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H164
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa23-211 from mouse TFPI2, expressed in NS0 cells (NP_033390). Species Sequence Homology: rat, 86%; human and chimpanzee, 58%; canine, 54%.
SpecificityRecognizes recombinant mouse TFPI-2 in Direct ELISA and Western Blot. No crossreactivity with recombinant mouse TFPI or recombinant human TFPI-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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