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You are here:Home » Antibodies » Antibodies-Protease Inhibitors » Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)

Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP
TFPI-2 is a secreted protein that inhibits several serine proteases.
Catalog #T5576-21
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml with appropriate secondary reagents detects mouse TFPI-2. The detection limit for recombinant mouse TFPI-2 is ~5ng/well.
Western Blot: 1-2ug/ml with the appropriate secondary reagents detects mouse TFPI-2. Using a colorimetric detection system, the detection limit for recombinant mouse TFPI-2 is ~10ng/lane under non-reducing conditions. Reducing conditions are not recommended for use.
Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant mouse TFPI-2 from conditioned cell culture medium.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H164
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa23-211 from mouse TFPI2, expressed in NS0 cells (NP_033390). Species Sequence Homology: rat, 86%; human and chimpanzee, 58%; canine, 54%.
SpecificityRecognizes recombinant mouse TFPI-2 in Direct ELISA and Western Blot. No crossreactivity with recombinant mouse TFPI or recombinant human TFPI-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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