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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)

Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP
TFPI-2 (tissue factor pathway inhibitor-2; also placental protein 5/PP5) is a secreted, presumably 27-34kD glycoprotein member of the Kunitz domain-containing proteinase inhibitor family. In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases. Mature mouse TFPI-2 is 208aa in length. It contains three Kunitz inhibitor domains, the most N-terminal of which (aa36-86) is crucial to inhibitor activity. Over aa23-211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively.
Catalog #T5576-21A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml. Detection limit for rmTFPI-2 is ~20 ng/lane and 2 ng/lane under non-reducing and reducing conditions.
ELISA (direct): 0.5-1ug/ml. Detection limit for rmTFPI-2 is ~0.1ng/well.
Immunoprecipitation: Immunoprecipitates rmTFPI-2 from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa 23-211 from recombinant mouse TFPI-2 expressed in NS0 cells (O35536).
SpecificityRecognizes mouse TFPI-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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