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You are here:Home » Antibodies » Antibodies-Protease Inhibitors » Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)

Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP
TFPI-2 (tissue factor pathway inhibitor-2; also placental protein 5/PP5) is a secreted, presumably 27-34kD glycoprotein member of the Kunitz domain-containing proteinase inhibitor family. In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases. Mature mouse TFPI-2 is 208aa in length. It contains three Kunitz inhibitor domains, the most N-terminal of which (aa36-86) is crucial to inhibitor activity. Over aa23-211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively.
Catalog #T5576-21A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml. Detection limit for rmTFPI-2 is ~20 ng/lane and 2 ng/lane under non-reducing and reducing conditions.
ELISA (direct): 0.5-1ug/ml. Detection limit for rmTFPI-2 is ~0.1ng/well.
Immunoprecipitation: Immunoprecipitates rmTFPI-2 from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa 23-211 from recombinant mouse TFPI-2 expressed in NS0 cells (O35536).
SpecificityRecognizes mouse TFPI-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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