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Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)
Anti -Tissue Factor Pathway Inhibitor 2 (TFPI2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IP |
|
| TFPI-2 (tissue factor pathway inhibitor-2; also placental protein 5/PP5) is a secreted, presumably 27-34kD glycoprotein member of the Kunitz domain-containing proteinase inhibitor family. In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases. Mature mouse TFPI-2 is 208aa in length. It contains three Kunitz inhibitor domains, the most N-terminal of which (aa36-86) is crucial to inhibitor activity. Over aa23-211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively. | | | Catalog # | T5576-21A | | Applications | Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml. Detection limit for rmTFPI-2 is ~20 ng/lane and 2 ng/lane under non-reducing and reducing conditions. | | ELISA (direct): 0.5-1ug/ml. Detection limit for rmTFPI-2 is ~0.1ng/well. | | Immunoprecipitation: Immunoprecipitates rmTFPI-2 from conditioned media of transfected NS0 cells. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Mouse | | Concentration | ~0.2mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by affinity chromatography. | | Immunogen | Recombinant protein corresponding to aa 23-211 from recombinant mouse TFPI-2 expressed in NS0 cells (O35536). | | Specificity | Recognizes mouse TFPI-2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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