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Timentin (Ticarcillin Disodium/Potassium Clavulanate 15:1)
Timentin (Ticarcillin Disodium/Potassium Clavulanate 15:1)
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| Catalog # | T5579 | | Timentin is a suitable antibiotic for Agrobacterium counterselection and a mixture of two antibiotics | ticarcillin and clavulanic acid. Most wild Agrobacterium isolates have beta-lactamase activity which clavulanic acid exhibits activity towards. | | Many Agrobacterium isolates are highly susceptible to Timentin. Agrobacterium strains carrying the standard pBR beta-lactamase gene are also Timentin (clavulanic acid) susceptible. Timentin killing of Agrobacterium wild-type strains is three logs greater than with comparable doses of carbenicillin. In plant transformation experiments, 0.1mg/ml Timentin (15 | 1) is sufficient to counterselect Agrobacterium. | | The appropriate dose in tissue culture medium is 0.1 mg/ml (not 0.1 mg/liter) of 15 | 1 mixture or 0.2 mg/ml of 30:1 mixture. To improve efficacy (of any antimicrobial), solid media are best slightly dried before use. At these concentrations, Timentin exhibits no phytotoxicity to | | Arabidopsis root cultures. | | Storage and Stability | Store at 4°C in hermetically sealed container, protected from light. Stable for 12 months at 4°C. | | CAS Number | 4697-14-7, 61177-45-5 | | Ticarcillin Disodium/Potassium Clavulanate =15 | 1 | | | Appearance | White to off-white to slight yellow powder | | | Identification (HPLC) | Positive | | | Solubility | | | Colorless, clear, complete | | | Moisture (Karl-Fischer) | 4.2% | | | pH | 5.5-7.5 | | | Assay (HPLC) | | | 750ug/mg (ticarcillin, on dried basis) | | 50ug/mg (clavulanic acid, on dried basis) | | | Related substances | | | -The largest impurity | 4.0% | | -The second impurity | 2.5% | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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