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You are here:Home » Biochemicals » Biochem-Antibiotics » Timentin (Ticarcillin Disodium/Potassium Clavulanate 15:1)

Timentin (Ticarcillin Disodium/Potassium Clavulanate 15:1)

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Specifications

Catalog #T5579
Timentin is a suitable antibiotic for Agrobacterium counterselection and a mixture of two antibiotics ticarcillin and clavulanic acid. Most wild Agrobacterium isolates have beta-lactamase activity which clavulanic acid exhibits activity towards.
Many Agrobacterium isolates are highly susceptible to Timentin. Agrobacterium strains carrying the standard pBR beta-lactamase gene are also Timentin (clavulanic acid) susceptible. Timentin killing of Agrobacterium wild-type strains is three logs greater than with comparable doses of carbenicillin. In plant transformation experiments, 0.1mg/ml Timentin (151) is sufficient to counterselect Agrobacterium.
The appropriate dose in tissue culture medium is 0.1 mg/ml (not 0.1 mg/liter) of 151 mixture or 0.2 mg/ml of 30:1 mixture. To improve efficacy (of any antimicrobial), solid media are best slightly dried before use. At these concentrations, Timentin exhibits no phytotoxicity to
Arabidopsis root cultures.
Storage and Stability Store at 4°C in hermetically sealed container, protected from light. Stable for 12 months at 4°C.
CAS Number4697-14-7, 61177-45-5
Ticarcillin Disodium/Potassium Clavulanate =151
AppearanceWhite to off-white to slight yellow powder
Identification (HPLC)Positive
Solubility
Colorless, clear, complete
Moisture (Karl-Fischer)4.2%
pH5.5-7.5
Assay (HPLC)
750ug/mg (ticarcillin, on dried basis)
50ug/mg (clavulanic acid, on dried basis)
Related substances
-The largest impurity4.0%
-The second impurity2.5%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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