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You are here:Home » BioSeparation? » BioSep-Assay Detection, Substrates » TMB Stable Liquid Substrate (1.13mM) for HRPO (3,3',5,5'-Tetramethylbenzidine)

TMB Stable Liquid Substrate (1.13mM) for HRPO (3,3',5,5'-Tetramethylbenzidine)


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TMB Stable Liquid Substrate is a peroxidase substrate which produces a stable insoluble aquamarine blue precipitate at the reaction site with little or no background. It is ideal for many immunoblotting procedures and can significantly increase the detection limits of assays on a variety of membranes.
Catalog #T6000
TMB, when reacted with horseradish peroxidase (HRP) and hydrogen peroxide, forms a blue free radical cation 1-electron oxidation product. It is routinely used in ELISA procedures. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate are usually added.
Lot-to-lot variations of molecular weights and degree of sulfonation of charged polysaccharides increase the complexity of manufacturing processes.
United States Biological has developed proprietary methodology that eliminates the use of sugar polymers and guarantees the production of a consistent single component precipitating TMB solution.
pH 4.9±0.03
Storage and StabilityStable for 12 months at RT. To extend the stability of product, refrigerate at 4°C.
CAS NumberN/A
Single component system
Room temperature stable
Exhibits low background levels
Safe to use - no weighing of hazardous powders
Save preparation time - use right out of the bottle
Ultra-low sensitivity
Minimal lot-to-lot variation
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names3,3?,5,5?-Tetramethylbenzidine; Precipitating

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