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You are here:Home » Antibodies » Abs to Vitamins » Anti -a-Tocopherol (Vitamin E), Conjugated

Anti -a-Tocopherol (Vitamin E), Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
alpha-Tocopherol is a form of vitamin E that is preferentially absorbed and accumulated in humans. The measurement of "vitamin E" activity in international units (IU) was based on fertility enhancement by the prevention of spontaneous abortions in pregnant rats relative to alpha-Tocopherol.
Catalog #T8025-05
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
ImmunogenSynthetic alpha-Tocopherol acid succinate, conjugated with BSA.
SpecificityRecognizes protein-linked alpha-Tocopherol (vitamin E). ELISA test results:
Compound Crossreactivity Ratio
a-Tocopherol succinate-BSA 1
Oleic acid-BSA 1/100
Myristic acid-BSA 1/100
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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