| Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate |
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| and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR1 is expressed at the cell surface as a heterodimer with either TLR2 or TLR6. These dimers recognize GPI-linked proteins and di- and triacyl lipoproteins. TLR4 and TLR5 are also expressed at the cell surface and respond to LPS and bacterial flagellin, respectively. TLRs 3, 7, 8 and 9 are localized in intracellular acidic compartments such as the phagolysosome (4) and recognize viral RNA and CpG DNA sequences. TLR10 is homologous to TLR1 and TLR6 and highly expressed in lymphoid tissues, although it exists only as a pseudogene in mice (5). |
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| Catalog # | T8049-95 |
| Kit Components | T8049-95A: TLR1 Antibody (NT), 1x50ug |
| T8049-95B: TLR2 Antibody (NT), 1x50ug |
| T8049-95C: TLR3 Antibody (CT), 1x50ug |
| T8049-95D: TLR4 Antibody (NT), 1x50ug |
| T8049-95E: TLR5 Antibody (CT), 1x50ug |
| T8049-95F: TLR6 Antibody (IN), 1x50ug |
| T8049-95G: TLR7 Antibody (IN), 1x50ug |
| T8049-95H: TLR8 Antibody (IN), 1x50ug |
| T8049-95J: TLR9 Antibody (IN), 1x50ug |
| T8049-95K: TLR10 Antibody (IN), 1x50ug |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-5ug/ml |
| Immunohistochemistry: 1-5ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Whole cell lysates |
| T8049-95A: mouse spleen lysate (for TLR1 antibody) |
| T8049-95B: A20 cell lysate (for TLR2 antibody) |
| T8049-95C Daudi cell lysate (for TLR3, TLR7, and TLR8 antibodies) |
| T8049-95D PC-3 cell lysate (for TLR4 antibody) |
| T8049-95E THP-1 cell lysate (for TLR5 antibody) |
| T8049-95F Jurkat cell lysate (for TLR6 and TLR9 antibodies) |
| T8049-95G Daudi cell lysate (for TLR3, TLR7, and TLR8 antibodies) |
| T8049-95H Daudi cell lysate (for TLR3, TLR7, and TLR8 antibodies) |
| T8049-95 J Jurkat cell lysate (for TLR6 and TLR9 antibodies) |
| T8049-95K human lymph node cell lysate (for TLR10 antibody) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | N/A |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptides corresponding to amino acid sequences from each of the corresponding proteins |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
