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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP IH FC

TLR2 is a 90kD type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes and is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.
Catalog #	T8050-09A
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry (frozen) and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:10- 1:50. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Hybridoma	Spleen cells from immunized Balb/c mice were fused with cells of the NSO myeloma cell line.
Recommended Positive Control Tissue	Tonsil
Recommended Negative Control	I1904-83P1: Mouse IgG2a
Recommended Secondary Reagents	F(ab')2 rabbit anti-mouse IgG FITC conjugate
	F(ab')2 Rabbit anti mouse IgG: RPE
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	3H3049
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified IgG
Immunogen	CHO-TLR2 cells.
Specificity	Recognizes human toll-like receptor 2 (TLR2). Species Crossreactivity: Cynomolgus monkey, rhesus monkey and marmoset.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.