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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR2 (Toll Like Receptor 2, CD282) (FITC)

Anti -TLR2 (Toll Like Receptor 2, CD282) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. T8050-09V is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human PBMCs.
Catalog #T8050-09V
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Functional Studies and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml showed a band at ~90kD in non-reduced peripheral blood mononuclear cells.
Immunohistochemistry (frozen): 1:50 for acetone fixed tissues using 3% H2O2 for blockade of endogenous peroxidases
Immunohistochemistry (paraffin): 1ug/ml for HOPE-fixed tissue for 16hr at 4°C.
Immunoprecipitation: T8050-09V conjugated Sepharose immunoprecipitated lysed monocytes
Immunofluorescence: 5-10ug for staining HMECs fixed with 4% paraformaldehyde in PBS, permeabilized and blocked.
Functional Studies: 1ug/5x10e7 blocks TNF-a release induced by bacteria from periperal blood mononuclear cells
Flow Cytometry: 2ug/3x10e5 untreated or 0.4% formaldehyde fixed cells. Permeabilized or 4% paraformaldehyde fixed cells are not recommended for this application.
Optimal dilutions to be determined by the researcher.
Positive ControlPeripheral blood mononuclear cells (PBMCs), granulocytes and monocytes.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9G278
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified.
ImmunogenRecombinant TLR-2 (CHO-TLR-2).
SpecificityRecognizes human Toll-like receptor 2 (TLR2, CD282). Species crossreactivity: cynomolgus monkey, rhesus monkey, marmosef monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD282, Toll-like receptor 2


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