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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E IH

Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.
Catalog #	T8050-10
	Human Toll Like Receptor-2 (TLR2) is also described asToll/Interluekin-1 Receptor-Like Gene 4 (TIL4).
Applications
	Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	ELISA: 1:50,000-1:100,000
Immunohistochemistry	1:250-1:500 (Human spleen).
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide CLEIDASDLQSYEPKSLKSIQNVSHLI corresponding to aa 179-204 of human TLR2.
Specificity	Recognizes human TLR2. Peptide sequence is < 50% identical to other human TLR receptors in this region. Not tested for crossreactivity to mouse TLR2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.