Anti -TLR2 (Toll-like Receptor 2, Toll/Interleukin-1 Receptor-like Protein 4, CD282, TIL4)
(FITC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP IH FC N |
|
| The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4). | | | Catalog # | T8050-12E | | Applications | Suitable for use in Flow Cytometry, Immunoprecipitation, Neutralization and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry (Cell Surface): 0.5ug/10e6 cells | | Immunohistochemistry: Frozen | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 11E369 | | Host | Mouse | | Source | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 10mM Tris, 150mM sodium chloride, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC). | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | The extracellular domain of mouse TLR2. | | Specificity | Recognizes mouse TLR2. Species Crossreactivity: human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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