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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR2 (Toll-like Receptor 2, Toll/Interleukin-1 Receptor-like Protein 4, CD282, TIL4)

Anti -TLR2 (Toll-like Receptor 2, Toll/Interleukin-1 Receptor-like Protein 4, CD282, TIL4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC FC IF
The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
Catalog #T8050-12P
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1-2ug/10e6 cells
Immunoprecipitation: 2-5ug
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.
Positive ControlPBMCs, Ramos
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No11E368
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenCHO cells transfected with human TLR2 cDNA.
SpecificityRecognizes human TLR2. Species Crossreactivity: canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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