Anti -TLR2 (Toll-like Receptor 2, Toll/Interleukin-1 Receptor-like Protein 4, CD282, TIL4)
(Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP IC FC IF |
|
| The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4). | | | Catalog # | T8050-12R | | Applications | Suitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry (Cell Surface): 2-5ug/1x10e6 cells | | Flow Cytometry (Intracellular): 2-5ug/1x10e6 cells | | Immunoprecipitation: 2-5ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | Ramos cells | | Storage and Stability | May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 11E368 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | CHO cells transfected with human TLR2 cDNA. | | Specificity | Recognizes human TLR2. Species Crossreactivity: canine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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